The Metabolic Integration Of Salmonella Enteric My First Research Experience

1303 Words Apr 12th, 2016 6 Pages
Research Experience

Metabolic Integration in Salmonella enterica: My first research experience began with a summer internship in the Bacteriology department at the University of Wisconsin, Madison, investigating the compensatory mechanisms involved in metabolic integration. The overarching goal was to identify the changes and alternative pathways required to sustain life in a Salmonella enterica strain whose thiamine (vitamin B1) biosynthetic gene, thiH, had been replaced by an equivalent non-homologous gene from Bacillus subtilis, thiO, which is more prevalent than thiH among bacteria. This strain grows normally in aerobic conditions with and without B1 supplementation. However, under anaerobic conditions growth occurs only in media supplemented with B1. My first project was to determine whether anaerobic growth without B1 could be restored by overexpressing thiO, which led to my first scientific achievement of successfully constructing an S. enterica strain with a promoter mutation to overexpress thiO. However, I found that overexpression of thiO does not reverse growth defects seen in anaerobic conditions. In my second project, I determined that leucine and valine are critical amino acids for restoring growth under both fermenting and anaerobic nutrient-deprived conditions. Lastly, I was responsible for isolating a mutant strain that spontaneously gained the ability to produce vitamin B1 in order to gain insight into genomic alterations that affect the bacteria’s…

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